pH is a critical parameter in method development and routine enzyme assay measurement. At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable ...
Acid phosphatase hydrolyses phosphate ester bonds under an optimum pH of 4.8 and an optimum temperature of 37 °C. In this experiment you will use an artificial substrate, para-nitrophenylphosphate ...
As with many chemical reactions, the rate of an enzyme-catalysed reaction increases as the temperature increases. However, at high temperatures the rate decreases again because the enzyme becomes ...
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